凝態物理專題演講
Advanced Topics on Condensed Matter Physics
Department of Physics, NCU
Least-Action Principle in Analyzing Mass Spectra of Virus Capsids, Biomarkers Finding of Mycobacterium Tuberculosis Complex, and Plasma for Charge Creation of Bacteria Particles
Speaker
Prof. Wen-Ping Peng
Department of Physics, National Dong Hwa University
Date 2015.10.22 (Thur)
Time 14:00
Place S4-625
Abstract:
In this talk, first I will address the action principle, S=, can be adopted to analyze complicated biological mass spectra, such as virus capsids. We constructed a minimum standard deviation theory to correctly assign charges of virus capsids and developed a game-theory-based search engine to define the boundaries of complicated electrospray mass spectra [1, 2]. Second, with nanodiamond nanotechnology and matrix-assisted laser desorption ionization (MALDI) time-of-flight (TOF) mass spectrometry technique, we found an early secreted cell filtrate protein 10 (CFP-10) could be treated as a biomarker of mycobacterium tuberculosis complex (MTC) [3, 4]. We confirmed that by detecting the singly charged species of CFP-10 antigen, the sensitivity and the specificity of MTC samples could reach 97.4% and 100%. This indicates CFP-10 antigen can be used as an early diagnosis biomarker in clinical practice. Finally, I will report for the first time that a laser-induced acoustic desorption plasma (LIADP) ion source can allow ionization of intact bacteria particles and gain charges. By combining charge detection quadrupole ion trap mass spectrometer (CD-QIT MS), the physical quantities of mass-to-charge ratio and charge of bacteria can be measured with high speed [5, 6]. This setup has potential to measure dangerous bacteria with safety.
References:
1.Tseng, Y. H.; Uetrecht, C.; Heck, A. J. R.; Peng, W. P.: Interpreting the Charge State Assignment in Electrospray Mass Spectra of Bioparticles. Anal. Chem. 2011, 83, 1960-1968.
2.Tseng, Y.-H.; Uetrecht, C.; Yang, S.-C.; Barendregt, A.; Heck, A. J. R.; Peng, W.-P.: Game-Theory-Based Search Engine to Automate the Mass Assignment in Complex Native Electrospray Mass Spectra. Anal. Chem. 2013, 85, 11275-11283.
3.Soo, P.-C.; Kung, C.-J.; Horng, Y.-T.; Chang, K.-C.; Lee, J.-J.; Peng, W.-P.: Detonation Nanodiamonds for Rapid Detection of Clinical Isolates of Mycobacterium tuberculosis Complex in Broth Culture Media. Anal. Chem. 2012, 84, 7972-7978.
4.Soo, P.-C.; Horng, Y.-T.; Chen, A.-T.; Yang, S.-C.; Chang, K.-C.; Lee, J.-J.; Peng, W.-P.: Validation of nanodiamond-extracted CFP-10 antigen as a biomarker in clinical isolates of Mycobacterium tuberculosis complex in broth culture media. Tuberculosis 95, 620-624.
5.Peng, W. P.; Lin, H. C.; Lin, H. H.; Chu, M.; Yu, A. L.; Chang, H. C.; Chen, C. H.: Charge-monitoring laser-induced acoustic desorption mass spectrometry for cell and microparticle mass distribution measurement. Angew Chem Int Edit 2007, 46, 3865-3869.
6.Peng, W.-P.; Yang, Y.-C.; Kang, M.-W.; Lee, Y. T.; Chang, H.-C.: Measuring Masses of Single Bacterial Whole Cells with a Quadrupole Ion Trap. Journal of the American Chemical Society 2004, 126, 11766-11767.