透過分形維度和熵分析評估斑馬魚在暴露於二十種抗生素後的運動複雜性
Antibiotics
Michael Edbert Suryanto, Chun-Chuen Yang, Gilbert Audira, Ross D. Vasquez, Marri Jmelou M. Roldan, Tzong-Rong Ger, Chung-Der Hsiao
Evaluation of Locomotion Complexity in Zebrafish after Exposure to Twenty Antibiotics by Fractal Dimension and Entropy Analysis
抗生素廣泛用於水產養殖，以防止細菌感染和疾病傳播。一些抗生素在水中的半衰期相對較長，可能會對目標魚類產生一些不利影響。本研究通過表型方法在行為水平上分析了抗生素對斑馬魚的潛在不利影響。在急性暴露於濃度為 100 ppb 的 20 種不同抗生素 10 天后，我們對成年斑馬魚進行了三維 (3D) 運動跟踪。通過分形維數和排列熵分析對它們的運動複雜性進行了分析和比較。通過結合從行為端點改變收集的數據來執行降維方法。主成分和層次分析得出的結論是，三種抗生素：阿莫西林、甲氧芐啶和泰樂菌素，表現出獨特的特徵。在後續研究中觀察到這三種抗生素在較低濃度（1 和 10 ppb）下的作用。根據結果，這些抗生素可以引發成年斑馬魚的一些行為改變，即使是低劑量也是如此。運動行為活動的顯著變化，如總距離活動、平均速度、快速運動時間、角速度、頂部/底部持續時間和蜿蜒運動與神經運動障礙、焦慮水平和壓力反應高度相關。這項研究提供了基於體內實驗的證據，以支持應仔細處理某些抗生素的使用這一觀點，因為它們會引起魚類行為改變的顯著影響。
Antibiotics are extensively used in aquaculture to prevent bacterial infection and the spread of diseases. Some antibiotics have a relatively longer half-life in water and may induce some adverse effects on the targeted fish species. This study analyzed the potential adverse effects of antibiotics in zebrafish at the behavioral level by a phenomic approach. We conducted three-dimensional (3D) locomotion tracking for adult zebrafish after acute exposure to twenty different antibiotics at a concentration of 100 ppb for 10 days. Their locomotor complexity was analyzed and compared by fractal dimension and permutation entropy analysis. The dimensionality reduction method was performed by combining the data gathered from behavioral endpoints alteration. Principal component and hierarchical analysis conclude that three antibiotics: amoxicillin, trimethoprim, and tylosin, displayed unique characteristics. The effects of these three antibiotics at lower concentrations (1 and 10 ppb) were observed in a follow-up study. Based on the results, these antibiotics can trigger several behavioral alterations in adult zebrafish, even in low doses. Significant changes in locomotor behavioral activity, such as total distance activity, average speed, rapid movement time, angular velocity, time in top/bottom duration, and meandering movement are highly related to neurological motor impairments, anxiety levels, and stress responses were observed. This study provides evidence based on an in vivo experiment to support the idea that the usage of some antibiotics should be carefully addressed since they can induce a significant effect of behavioral alterations in fish.