細胞也會被拉高！揭開反覆拉扯如何塑造柱狀細胞的祕密
Cell Reports
Lun-Wei Lee, Gang-Hui Lee, I-Hsiu Su, Chia-Hsuan Lu, Keng-Hui Lin, Fu-Lai Wen, Ming-Jer Tang
Mechanobiological mechanism of cyclic stretch-induced cell columnarization
本研究發現，長時間單軸循環拉伸，會誘發上皮細胞內部細胞骨架重塑與細胞間黏著分子重組，促使MDCK細胞(一種狗腎上皮細胞)由立方形轉變為柱狀形態。透過原子力顯微鏡的量測與數學模型的分析，我們證實此過程伴隨著細胞頂端與底端的張力增強，因而驅動細胞形狀改變。此研究揭示上皮細胞適應機械刺激之力學調控機制
This study demonstrates that long-term uniaxial cyclic stretching induces cytoskeletal remodeling and reorganization of cell–cell adhesion molecules in epithelial cells, driving the morphological transition of MDCK cells (a canine kidney epithelial cell line) from a cuboidal to a columnar shape. Through atomic force microscopy (AFM) measurements and mathematical modeling analyses, we confirmed that this process is accompanied by increased tensile forces at both the apical and basal regions of the cell, which in turn promote the shape change. Our findings reveal the mechanobiological mechanisms by which epithelial cells adapt to mechanical stimulation.